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Categories containing this gene/protein


phosphotransferase systems, utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons


AbrB regulon, CcpA regulon, GmuR regulon

Gene


  • Coordinates on the chromosome (coding sequence): 626,933 -> 627,265
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • PTS permease, lactose permease (Lac) family PubMed
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 3K1S (IIA(Cel) from B. anthracis, 42% identity)
  • Localization

    Interactions

  • GmuA-HPr, GmuA-GmuB
  • Additional information

    Expression and Regulation


    Operon

  • gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induced by conjac glucomannan (requires GmuG) (GmuR) PubMed
  • induced by cellobiose (GmuR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed
  • Regulatory mechanism

  • GmuR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-C189 (ydhN::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Chumsakul O, Takahashi H, Oshima T, Hishimoto T, Kanaya S, Ogasawara N, Ishikawa S

    Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation

    Nucleic Acids Res. 2011 Jan;39(2):414-28. doi: 10.1093/nar/gkq780. Epub 2010 Sep 3. PubMed PMID: 20817675; PubMed Central PMCID: PMC3025583.
    Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K

    Glucomannan utilization operon of Bacillus subtilis

    FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x. PubMed PMID: 18177310.
    Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K

    Glucomannan utilization operon of Bacillus subtilis

    FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x. PubMed PMID: 18177310.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
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