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Categories containing this gene/protein
utilization of specific carbon sources, phosphoproteins
This gene is a member of the following regulons
AbrB regulon, CcpA regulon, GlpP regulon
Coordinates on the chromosome (coding sequence): 1,003,344 -> 1,004,834
Catalyzed reaction/ biological activityATP + glycerol = ADP + sn-glycerol 3-phosphate (according to Swiss-Prot)
Protein familyFGGY kinase family (according to Swiss-Prot)
Effectors of protein activity
Structure3H46 (from Enterococcus casseliflavus, complex with glycerol) PubMedPtsH-GlpK
Expression and Regulation
OperonglpF-glpK PubMedSigA PubMed
Regulationrepressed by glucose (8-fold) (CcpA) PubMedinduction by glycerol (GlpP) PubMed
Regulatory mechanismCcpA: transcription repression PubMedGlpP-dependent RNA switch (transcriptional antitermination) PubMedAbrB: transcription activation PubMed
MutantBKE09290 (glpK::ermC) (available at the BGSC and in Jörg Stülke's lab)GP1865 (glpK::ermC) (available in Jörg Stülke's lab)
Labs working on this gene/protein
Josef Deutscher, Paris-Grignon, France
Chumsakul O, Takahashi H, Oshima T, Hishimoto T, Kanaya S, Ogasawara N,
Genome-wide binding profiles of the Bacillus subtilis transition
state regulator AbrB and its homolog Abh reveals their interactive role in
transcriptional regulation Nucleic Acids Res. 2011 Jan;39(2):414-28. doi:
10.1093/nar/gkq780. Epub 2010 Sep 3. PubMed PMID: 20817675; PubMed Central PMCID:
Yeh JI, Kettering R, Saxl R, Bourand A, Darbon E, Joly N, Briozzo P, Deutscher
Structural characterizations of glycerol kinase: unraveling
phosphorylation-induced long-range activation Biochemistry. 2009 Jan
20;48(2):346-56. doi: 10.1021/bi8009407. PubMed PMID: 19102629; PubMed Central
Darbon E, Servant P, Poncet S, Deutscher J
Antitermination by GlpP,
catabolite repression via CcpA and inducer exclusion triggered by P-GlpK
dephosphorylation control Bacillus subtilis glpFK expression Mol Microbiol. 2002
Feb;43(4):1039-52. PubMed PMID: 11929549.
Charrier V, Buckley E, Parsonage D, Galinier A, Darbon E, Jaquinod M, Forest
E, Deutscher J, Claiborne A
Cloning and sequencing of two enterococcal glpK
genes and regulation of the encoded glycerol kinases by
phosphoenolpyruvate-dependent, phosphotransferase system-catalyzed
phosphorylation of a single histidyl residue J Biol Chem. 1997 May
30;272(22):14166-74. PubMed PMID: 9162046.
Wehtje C, Beijer L, Nilsson RP, Rutberg B
Mutations in the glycerol kinase
gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on
glycerol Microbiology. 1995 May;141 ( Pt 5):1193-8. PubMed PMID: 7773413.
Beijer L, Rutberg L
Utilisation of glycerol and glycerol 3-phosphate is
differently affected by the phosphotransferase system in Bacillus subtilis FEMS
Microbiol Lett. 1992 Dec 15;100(1-3):217-20. PubMed PMID: 1335945.