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Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

The GlcT regulon


  • Coordinates on the chromosome (coding sequence): 1,456,092 -> 1,456,958
  • The protein

    Catalyzed reaction/ biological activity

  • [PRD-containing transcription factors] , RNA-binding protein, binds the ptsG RAT sequence
  • Protein family

  • [PRD-containing transcription factors] of the BglG/ SacY family
  • Paralogous protein(s)

  • LicT, SacT, SacY
  • Kinetic information


  • RNA-binding domain (N-terminal, constitutive antiterminator)
  • 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)
  • Modification

  • phosphorylation (His104)
  • Cofactors

    Effectors of protein activity


  • 3GWH (PRD-II) PubMed
  • 3RIO (RBD-PRD-I) PubMed
  • Localization


  • PtsH-GlcT
  • Additional information

    Expression and Regulation


    Sigma factor


    Regulatory mechanism

    Additional information

    Biological materials


  • available in Jörg Stülke's lab:
  • GP109 (in frame deletion)
  • GP777 (replacement of glcT and the ptsG-ptsH-ptsI operon by a spc cassette), PubMed
  • GP926 (substitution of glcT and ptsG by a tet cassette), PubMed
  • Expression vector

  • pGP124 (full length, in pWH844), available in Jörg Stülke's lab
  • pGP114 (amino acids 1-60, RNA-binding domain, in pWH844), available in Jörg Stülke's lab
  • pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in pWH844), available in Jörg Stülke's lab
  • pGP164 (both PRDs, in pWH844), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in pWH844), available in Jörg Stülke's lab
  • pGP424 (PRDI, in pWH844), available in Jörg Stülke's lab
  • pGP425 (PRDII, in pWH844), available in Jörg Stülke's lab
  • pGP442 (PRDI, in pGP570, with thrombin cleavage site), available in Jörg Stülke's lab
  • pGP443 (PRDII, in pGP570, with thrombin cleavage site), available in Jörg Stülke's lab
  • pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in pGP574), available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

  • GP1224 (spc, based on pGP1870), available in Jörg Stülke's lab
  • two-hybrid system

    FLAG-tag construct

  • GP1220 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein

  • Jörg Stülke, University of Göttingen, Germany
  • Homepage
  • References


    Commichau FM, Stülke J

    Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression

    Mol Microbiol. 2008 Feb;67(4):692-702. Epub 2007 Dec 11. Review. PubMed PMID: 18086213.
    Stülke J, Arnaud M, Rapoport G, Martin-Verstraete I

    PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria

    Mol Microbiol. 1998 Jun;28(5):865-74. Review. PubMed PMID: 9663674.

    Original publications

    Himmel S, Grosse C, Wolff S, Schwiegk C, Becker S

    Structure of the RBD-PRDI fragment of the antiterminator protein GlcT

    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):751-6. doi: 10.1107/S1744309112020635. Epub 2012 Jun 22. PubMed PMID: 22750856; PubMed Central PMCID: PMC3388913.
    Himmel S, Zschiedrich CP, Becker S, Hsiao HH, Wolff S, Diethmaier C, Urlaub H, Lee D, Griesinger C, Stülke J

    Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: functionality and phosphorylation specificity depend on the arrangement of the regulatory domains

    J Biol Chem. 2012 Aug 10;287(33):27731-42. doi: 10.1074/jbc.M112.388850. Epub 2012 Jun 21. PubMed PMID: 22722928; PubMed Central PMCID: PMC3431645.
    Himmel S, Wolff S, Becker S, Lee D, Griesinger C

    Detection and identification of protein-phosphorylation sites in histidines through HNP correlation patterns

    Angew Chem Int Ed Engl. 2010 Nov 15;49(47):8971-4. doi: 10.1002/anie.201003965. PubMed PMID: 20939030.
    Rodríguez DD, Grosse C, Himmel S, González C, de Ilarduya IM, Becker S, Sheldrick GM, Usón I

    Crystallographic ab initio protein structure solution below atomic resolution

    Nat Methods. 2009 Sep;6(9):651-3. doi: 10.1038/nmeth.1365. Epub 2009 Aug 16. PubMed PMID: 19684596.
    Schilling O, Herzberg C, Hertrich T, Vörsmann H, Jessen D, Hübner S, Titgemeyer F, Stülke J

    Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples

    Nucleic Acids Res. 2006;34(21):6102-15. Epub 2006 Oct 29. PubMed PMID: 17074746; PubMed Central PMCID: PMC1635312.
    Schilling O, Langbein I, Müller M, Schmalisch MH, Stülke J

    A protein-dependent riboswitch controlling ptsGHI operon expression in Bacillus subtilis: RNA structure rather than sequence provides interaction specificity

    Nucleic Acids Res. 2004 May 20;32(9):2853-64. Print 2004. PubMed PMID: 15155854; PubMed Central PMCID: PMC419612.
    Schmalisch MH, Bachem S, Stülke J

    Control of the Bacillus subtilis antiterminator protein GlcT by phosphorylation

    Elucidation of the phosphorylation chain leading to inactivation of GlcT. J Biol Chem. 2003 Dec 19;278(51):51108-15. Epub 2003 Oct 3. PubMed PMID: 14527945.
    Greenberg DB, Stülke J, Saier MH Jr

    Domain analysis of transcriptional regulators bearing PTS regulatory domains

    Res Microbiol. 2002 Oct;153(8):519-26. PubMed PMID: 12437213.
    Stülke J, Martin-Verstraete I, Zagorec M, Rose M, Klier A, Rapoport G

    Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT

    Mol Microbiol. 1997 Jul;25(1):65-78. PubMed PMID: 11902727.
    Langbein I, Bachem S, Stülke J

    Specific interaction of the RNA-binding domain of the bacillus subtilis transcriptional antiterminator GlcT with its RNA target, RAT

    J Mol Biol. 1999 Nov 5;293(4):795-805. PubMed PMID: 10543968.
    Bachem S, Stülke J

    Regulation of the Bacillus subtilis GlcT antiterminator protein by components of the phosphotransferase system

    J Bacteriol. 1998 Oct;180(20):5319-26. PubMed PMID: 9765562; PubMed Central PMCID: PMC107579.
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