SubtiBank SubtiBank

Categories containing this gene/protein

DEAD-box RNA helicases, translation, cold stress proteins, membrane proteins

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 511,106 -> 512,641
  • Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) PubMed
  • reduced number of ribosomes PubMed
  • no expression of the frlB-frlO-frlN-frlM-frlD operon PubMed
  • strongly increased expression of the ysbA-ysbB operon PubMed
  • transcription profile resulting from rny depletion: GEO PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for ribosome assembly (biogenesis of the large subunit) PubMed
  • Protein family

  • helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicases
  • Paralogous protein(s)

  • CshB, DeaD, YfmL
  • Kinetic information




    Effectors of protein activity



  • cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed
  • Interactions

  • CshA interacts with the RNA polymerase PubMed
  • part of the RNA degradosome PubMed
  • CshA-CshA PubMed
  • CshA-CspB PubMed
  • CshA-PnpA PubMed
  • CshA-Rny PubMed
  • CshA-RnjA PubMed
  • CshA-Eno PubMed
  • CshA-PfkA PubMed
  • DeaD-CshA PubMed
  • RnpA-CshA PubMed
  • RplA-CshA PubMed
  • RplC-CshA PubMed
  • Additional information

    Expression and Regulation


    Sigma factor


  • induced by cold shock PubMed
  • constitutive, similar expression at high and low temperatures, throughout growth and in both minimal and complex medium PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


  • GP1035 (aphA3), available in Jörg Stülke's lab
  • GP1083 (cat), available in Jörg Stülke's lab
  • Expression vector

  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

  • pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
  • B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,
  • GP1721 (in pBP43), expression of cshA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1074 (tet), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein

  • Mohamed Marahiel, Marburg University, Germany homepage
  • References

    Ogura M, Asai K

    Glucose Induces ECF Sigma Factor Genes, sigX and sigM, Independent of Cognate Anti-sigma Factors through Acetylation of CshA in Bacillus subtilis

    Front Microbiol. 2016 Nov 29;7:1918. eCollection 2016. PubMed PMID: 27965645; PubMed Central PMCID: PMC5126115.
    Cascante-Estepa N, Gunka K, Stülke J

    Localization of Components of the RNA-Degrading Machine in Bacillus subtilis

    Front Microbiol. 2016 Sep 21;7:1492. eCollection 2016. PubMed PMID: 27708634; PubMed Central PMCID: PMC5030255.
    Lehnik-Habrink M, Rempeters L, Kovács ÁT, Wrede C, Baierlein C, Krebber H, Kuipers OP, Stülke J

    DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other

    J Bacteriol. 2013 Feb;195(3):534-44. doi: 10.1128/JB.01475-12. Epub 2012 Nov 21. PubMed PMID: 23175651; PubMed Central PMCID: PMC3554002.
    Lehnik-Habrink M, Newman J, Rothe FM, Solovyova AS, Rodrigues C, Herzberg C, Commichau FM, Lewis RJ, Stülke J

    RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli

    J Bacteriol. 2011 Oct;193(19):5431-41. doi: 10.1128/JB.05500-11. Epub 2011 Jul 29. PubMed PMID: 21803996; PubMed Central PMCID: PMC3187381.
    Lehnik-Habrink M, Pförtner H, Rempeters L, Pietack N, Herzberg C, Stülke J

    The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex

    Mol Microbiol. 2010 Aug;77(4):958-71. doi: 10.1111/j.1365-2958.2010.07264.x. Epub 2010 Jun 21. PubMed PMID: 20572937.
    Hunger K, Beckering CL, Wiegeshoff F, Graumann PL, Marahiel MA

    Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis

    J Bacteriol. 2006 Jan;188(1):240-8. PubMed PMID: 16352840; PubMed Central PMCID: PMC1317592.

    CshA in other organisms

    Oun S, Redder P, Didier JP, François P, Corvaglia AR, Buttazzoni E, Giraud C, Girard M, Schrenzel J, Linder P

    The CshA DEAD-box RNA helicase is important for quorum sensing control in Staphylococcus aureus

    RNA Biol. 2013 Jan;10(1):157-65. doi: 10.4161/rna.22899. Epub 2012 Dec 10. PubMed PMID: 23229022; PubMed Central PMCID: PMC3590232.
    Roux CM, DeMuth JP, Dunman PM

    Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex

    J Bacteriol. 2011 Oct;193(19):5520-6. doi: 10.1128/JB.05485-11. Epub 2011 Jul 15. PubMed PMID: 21764917; PubMed Central PMCID: PMC3187390.
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