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Categories containing this gene/protein


phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons


CcpA regulon, LicT regulon

Gene


  • Coordinates on the chromosome (coding sequence): 4,033,778 -> 4,035,607
  • The protein


    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family

  • PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

  • cell membrane PubMed
  • Interactions

  • BglP-HPr, BglP-LicT
  • Additional information

    Expression and Regulation


    Operon

  • bglP-bglH-yxiE PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed by glucose (CcpA, LicT) PubMed
  • induced by salicin (LicT) PubMed
  • Regulatory mechanism

  • CcpA: transcription repression, (CcpA binding site overlaps -35 region) PubMed
  • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr PubMed
  • Additional information

  • An antisense RNA is predicted for bglP PubMed
  • Biological materials


    Mutant

  • GP475 (erm), available in the Stülke lab
  • Expression vector

  • pGP1290 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab,
  • pGP1300 (expression of bglP in B. subtilis, in pBQ200), available in Stülke lab
  • lacZ fusion

  • pGP600 (in pAC6), available in Stülke lab
  • GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Rothe FM, Wrede C, Lehnik-Habrink M, Görke B, Stülke J

    Dynamic localization of a transcription factor in Bacillus subtilis: the LicT antiterminator relocalizes in response to inducer availability

    J Bacteriol. 2013 May;195(10):2146-54. doi: 10.1128/JB.00117-13. Epub 2013 Mar 8. PubMed PMID: 23475962; PubMed Central PMCID: PMC3650534.
    Marciniak BC, Pabijaniak M, de Jong A, Dűhring R, Seidel G, Hillen W, Kuipers OP

    High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    BMC Genomics. 2012 Aug 17;13:401. doi: 10.1186/1471-2164-13-401. PubMed PMID: 22900538; PubMed Central PMCID: PMC3463425.
    Irnov I, Sharma CM, Vogel J, Winkler WC

    Identification of regulatory RNAs in Bacillus subtilis

    Nucleic Acids Res. 2010 Oct;38(19):6637-51. doi: 10.1093/nar/gkq454. Epub 2010 Jun 4. PubMed PMID: 20525796; PubMed Central PMCID: PMC2965217.
    Ollinger J, Song KB, Antelmann H, Hecker M, Helmann JD

    Role of the Fur regulon in iron transport in Bacillus subtilis

    J Bacteriol. 2006 May;188(10):3664-73. PubMed PMID: 16672620; PubMed Central PMCID: PMC1482855.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
    Krüger S, Gertz S, Hecker M

    Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression

    J Bacteriol. 1996 May;178(9):2637-44. PubMed PMID: 8626332; PubMed Central PMCID: PMC177989.
    Le Coq D, Lindner C, Krüger S, Steinmetz M, Stülke J

    New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog

    J Bacteriol. 1995 Mar;177(6):1527-35. PubMed PMID: 7883710; PubMed Central PMCID: PMC176769.
    Krüger S, Hecker M

    Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis

    J Bacteriol. 1995 Oct;177(19):5590-7. PubMed PMID: 7559347; PubMed Central PMCID: PMC177369.
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